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Determination of triamcinolone in human plasma by a sensitive HPLC-ESI-MS/MS method: application for a pharmacokinetic study using nasal spray formulation.

Author(s): Cesar IC, Byrro RM, de Santana e Silva Cardoso FF, Mundim IM, de Souza Teixeira L, de Sousa WC, Gomes SA, Bellorio KB, Bretas JM, Pianetti GA

Affiliation(s): Instituto de Ciencias Farmaceuticas, Alameda Coronel Eugenio Jardim 53, 74175-100 Goiania, GO, Brazil. isaccesar@bol.com.br

Publication date & source: 2011-03, J Mass Spectrom., 46(3):320-6.

A liquid chromatography-electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS) method for the quantitation of triamcinolone in human plasma after nasal spray application was developed and validated. Betamethasone was used as internal standard (IS). The analytes were extracted by a liquid-liquid procedure and separated on a Zorbax Eclipse XDB C(18) column with a mobile phase composed of 2 mM aqueous ammonium acetate pH 3.2 and acetonitrile (55:45). Selected reaction monitoring was performed using the transitions m/z 435 --> 415 and m/z 393 --> 373 to quantify triamcinolone acetonide and betamethasone, respectively. Calibration curve was constructed over the range of 20-2000 pg/ml for triamcinolone acetonide. The lower limit of quantitation was 20 pg/ml. The mean RSD values were 4.6% and 5.7% for the intra-run and inter-run precision, respectively. The mean accuracy value was 98.5% and a recovery rate corresponding to 97.5% was achieved. No matrix effect was detected in the samples. The validated method was successfully applied to determine the plasma concentrations of triamcinolone acetonide in healthy volunteers, in a pharmacokinetic study with nasal spray formulation. Copyright (c) 2011 John Wiley & Sons, Ltd.

Page last updated: 2011-12-09

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