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Cryotherapy and GM-CSF in Treating Patients With Lung Metastases or Primary Lung Cancer

Information source: Barbara Ann Karmanos Cancer Institute
ClinicalTrials.gov processed this data on August 23, 2015
Link to the current ClinicalTrials.gov record.

Condition(s) targeted: Kidney Cancer; Lung Cancer; Metastatic Cancer; Unspecified Adult Solid Tumor, Protocol Specific

Intervention: sargramostim (Biological); flow cytometry (Other); immunoenzyme technique (Other); biopsy (Procedure); cryosurgery (Procedure)

Phase: Phase 2

Status: Completed

Sponsored by: Barbara Ann Karmanos Cancer Institute

Official(s) and/or principal investigator(s):
Peter J. Littrup, MD, Study Chair, Affiliation: Barbara Ann Karmanos Cancer Institute


RATIONALE: Cryotherapy kills tumor cells by freezing them. Giving an injection of GM-CSF before cryotherapy and inhaling GM-CSF after cryotherapy may interfere with the growth of tumor cells and shrink the tumor. Giving cryotherapy together with GM-CSF may kill more tumor cells. PURPOSE: This phase II trial is studying how well giving cryotherapy together with GM-CSF works in treating patients with lung metastases or primary lung cancer.

Clinical Details

Official title: Percutaneous Cryotherapy and Aerosolized GM-CSF for Pulmonary Metastases and Primary Lung Cancer

Study design: Intervention Model: Single Group Assignment, Masking: Open Label, Primary Purpose: Treatment

Primary outcome: Immunologic Response as Measured by ELISPOT Assay and Flow Cytometry

Secondary outcome:

Clinical Response as Measured by CT Criteria


Immune Function and Cancer-specific Response

Detailed description: OBJECTIVES: Primary

- Determine whether percutaneous cryotherapy in combination with aerosolized sargramostim

(GM-CSF) has any demonstrable immunologic effect in patients with pulmonary metastases or primary lung cancer.

- Determine whether any systemic immune response is detectable by the combination of

cryotherapy as the antigen presentation source and GM-CSF as the immunologic adjuvant.

- Determine whether low morbidities will be maintained in patients treated with this


- Determine whether effective immunization is associated with a drop in CD4+, CD25+,

LTP(TGF-β1)+, Tr cells as measured by flow cytometry or ELISPOT assay for TGF-β1-secreting cells. Secondary

- Determine clinical response (i. e., tumor control in the dominant masses undergoing

cryotherapy or in other metastatic sites) as measured by CT criteria.

- Determine the toxicity of this regimen in these patients.

OUTLINE: Patients undergo CT-guided core biopsy of a dominant lung mass and placement of at least 2 cryoprobes. Prior to initiating the freeze, patients receive an interstitial injection of sargramostim (GM-CSF) near the tumor. Patients then undergo percutaneous cryotherapy over 2 hours utilizing a freeze-thaw-freeze cycle. Beginning within 3 days of cryotherapy, patients receive aerosolized GM-CSF twice daily for 1 week. Beginning on day 32, patients may elect to undergo a second course of treatment as described above in the absence of disease progression or unacceptable toxicity. Patients undergo blood and tumor tissue collection at baseline and periodically during study for immunological correlative studies. Peripheral blood mononuclear cells isolated from blood samples are analyzed for antigen-specific CD4-positive or CD8-positive T-cell response by flow cytometry or by TGF-β1 ELISPOT assay to measure TGF-β1- secreting cells. Tumor cell lysates extracted from tumor samples are pulsed with autologous dendritic cells and analyzed by ELISPOT assay to measure T-cell reactivity in tumor specimens. After completion of study therapy, patients are followed at 6 and 12 months.


Minimum age: 18 Years. Maximum age: N/A. Gender(s): Both.



- Histologically confirmed diagnosis of 1 of the following:

- Primary non-small cell lung cancer (NSCLC)

- Any stage nonoperative NSCLC or patient refuses surgery

- Any cancer with pulmonary metastatic disease (including renal cell cancer)

- Stage IV disease (any T, any N, M1)

- Must have 1-10 pulmonary or mediastinal masses meeting the following criteria:

- At least 1 mass is appropriate for 2 sessions of core biopsy and cryotherapy

with relatively easy access/low risk in nonoperative patients (or those refusing surgery)

- The two dominant masses are defined as either the largest and/or those that may

cause imminent morbidity from continued local progression, thereby potentially benefiting from thoracic cryotherapy alone

- Optimal tumor size > 1. 0 cm

- Dominant masses up to 6 cm in diameter may be considered if thorough

cryotherapy coverage can be anticipated with minimal additional treatment morbidity

- Measurable disease, defined as tridimensional measurements of up to 6 different

pulmonary or mediastinal masses ≥ 0. 5 cm by CT scan

- No active pleural effusion that could be related to respiratory infection or requires

further work-up

- No untreated and/or unstable brain metastases


- Karnofsky performance status 70-100%

- Life expectancy ≥ 12 weeks

- Granulocyte count ≥ 1,500/mm³

- Platelet count ≥ 50,000/mm³

- INR < 1. 5 (i. e., normal PT/PTT)

- Hemoglobin ≥ 8. 0 g/dL

- Bilirubin ≤ 2 times upper limit of normal (ULN)

- AST ≤ 3 times ULN

- Satisfactory pulmonary function test as determined by supervising oncologist,

thoracic surgeon, or pulmonologist

- Not pregnant or lactating

- Negative pregnancy test

- Fertile patients must use effective contraception

- No other active malignancy except nonmelanoma skin cancer or carcinoma in situ of the


- Inactive history of cancer allowed if the patient has been disease-free for > 2


- No serious medical or psychiatric illnesses that would preclude informed consent or

limit survival to < 12 wks

- No uncontrollable cough or inability to lie flat

- No New York Heart Association class III or IV heart disease

- No known immunodeficiency state

- No uncontrolled infection

- No uncontrolled coagulopathy or bleeding diathesis

- No advance directive that would prevent the investigator from treating the

participant in the event of a complication occurring during or after the procedure

- No medical contraindication or potential problem that would preclude protocol


- More than 4 weeks since prior biologic therapy

- More than 4 weeks since prior immunotherapy

- More than 4 weeks since prior filgrastim (G-CSF) or sargramostim (GM-CSF)

- More than 4 weeks since prior chemotherapy (6 weeks for nitrosoureas or mitomycin)

- More than 4 weeks since prior radiotherapy

- More than 2 weeks since prior corticosteroids

- More than 1 week since prior parenteral antibiotics

- At least 1 week since prior aspirin or aspirin-like medications

- At least 3 days since prior warfarin, clopidogrel bisulfate, or similar compounds

- No concurrent GM-CSF other than study drug

- No concurrent G-CSF

- No concurrent radiotherapy

- No concurrent glucocorticosteroids

- No concurrent parenteral antibiotics

- No concurrent immunosuppressive agents

- No concurrent drugs that cause bleeding tendencies

- No other concurrent biologic therapy, immunotherapy, radiotherapy, or chemotherapy

Locations and Contacts

Barbara Ann Karmanos Cancer Institute, Detroit, Michigan 48201-1379, United States
Additional Information

Clinical trial summary from the National Cancer Institute's PDQ® database

Starting date: January 2006
Last updated: February 9, 2015

Page last updated: August 23, 2015

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