Amino Acids Expression Profiling of Patients With Sepsis
Information source: Chinese PLA General Hospital
ClinicalTrials.gov processed this data on August 23, 2015
Link to the current ClinicalTrials.gov record.
Condition(s) targeted: Sepsis; Amino Acids
Phase: N/A
Status: Active, not recruiting
Sponsored by: Chinese PLA General Hospital Official(s) and/or principal investigator(s):
Lixin Xie, Doctor, Study Director, Affiliation: Department of Respiratory Diseases, Chinese PLA General Hospital
Summary
The patients with sepsis are in the state of hypermetabolism, increased resting energy
expenditure, protein and fat catabolism disorder, negative nitrogen balance, insulin
resistance, hyperglycemia, and amino acid metabolism disorders. However, it is remain
unclear the changes of amino acids expression profiling in sepsis patients. In this study,
the investigators has planned to enroll 100 subjects, including 20 cases with systemic
inflammatory response syndrome (SIRS), 20 cases with sepsis, 20 cases with severe sepsis and
20 with septic shock. In addition, this study also include 20 normal cases as control. The
serum sample of patients with sepsis is draw on days 1, 3, 5, 7, 10, and 14 after first ICU
admission. High-performance liquid chromatography and tandem mass spectrometry was used to
detect the quantification of amino acids. The amino acids expression profiling contain
Arginine, Ornithine, Histidine, Cystine, Isoleucine, Cystathionine, Leucine, Homocystine,
Lysine, α-Amino-n-Butyric Acid, Methionine, Alanine, Phenylalanine, Anserine, Threonine,
β-Alanine, Tryptophan, β-Amino-Isobutyric Acid, Valine, Carnosine, γ-Amino-n-Butyric Acid,
Ethanolamine, Glycine, δ-Hydroxylysine, Serine, Hydroxy-L-Proline, Taurine,
1-Methyl-L-Histidine, Tyrosine, 3-Methyl-L-Mistidine, α-Amino-Adipic, Phospho-Ethanolamine,
Asparagine, Phospho-L-Serine, Aspartic acid, Proline, Citrulline, Sarcosine, Glutamic acid,
Argininosuccinic Acid, Glutamine, Homocitrulline. The investigators speculate that
measurement of amino acids expression profiling could be taken as an indicator for
assessment in critically ill patients.
Clinical Details
Official title: Amino Acids Expression Profiling of Patients With Sepsis
Study design: Observational Model: Case Control, Time Perspective: Retrospective
Primary outcome: Patients Outcome
Eligibility
Minimum age: 18 Years.
Maximum age: N/A.
Gender(s): Both.
Criteria:
Inclusion Criteria:
1. Male and female aged 18 years old and over;
2. Clinically confirmed infection;
3. Fulfilled at least two criteria of systemic inflammatory response syndrome (a) core
temperature higher than 38 °C or lower than 36 °C (b)respiratory rate above 20/min,
or PCO2 below 32 mmHg (c) pulse rate above 90/min, and (d) white blood cell count
greater than 12,000/μl or lower than < 4,000/μl or less than 10% of bands.
Exclusion Criteria:
1. parenteral nutrition;
2. diabetes and other metabolic-related diseases;
3. chronic liver disease;
4. neutropenia (≤ 500 neutrophils/mm3);
5. HIV infection;
6. patients or their relatives refused.
Locations and Contacts
Chinese PLA General Hospital, Beijing, Beijing 100853, China
Additional Information
Starting date: March 2012
Last updated: March 23, 2013
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