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Amino Acids Expression Profiling of Patients With Sepsis

Information source: Chinese PLA General Hospital
ClinicalTrials.gov processed this data on August 23, 2015
Link to the current ClinicalTrials.gov record.

Condition(s) targeted: Sepsis; Amino Acids

Phase: N/A

Status: Active, not recruiting

Sponsored by: Chinese PLA General Hospital

Official(s) and/or principal investigator(s):
Lixin Xie, Doctor, Study Director, Affiliation: Department of Respiratory Diseases, Chinese PLA General Hospital


The patients with sepsis are in the state of hypermetabolism, increased resting energy expenditure, protein and fat catabolism disorder, negative nitrogen balance, insulin resistance, hyperglycemia, and amino acid metabolism disorders. However, it is remain unclear the changes of amino acids expression profiling in sepsis patients. In this study, the investigators has planned to enroll 100 subjects, including 20 cases with systemic inflammatory response syndrome (SIRS), 20 cases with sepsis, 20 cases with severe sepsis and 20 with septic shock. In addition, this study also include 20 normal cases as control. The serum sample of patients with sepsis is draw on days 1, 3, 5, 7, 10, and 14 after first ICU admission. High-performance liquid chromatography and tandem mass spectrometry was used to detect the quantification of amino acids. The amino acids expression profiling contain Arginine, Ornithine, Histidine, Cystine, Isoleucine, Cystathionine, Leucine, Homocystine, Lysine, α-Amino-n-Butyric Acid, Methionine, Alanine, Phenylalanine, Anserine, Threonine, β-Alanine, Tryptophan, β-Amino-Isobutyric Acid, Valine, Carnosine, γ-Amino-n-Butyric Acid, Ethanolamine, Glycine, δ-Hydroxylysine, Serine, Hydroxy-L-Proline, Taurine, 1-Methyl-L-Histidine, Tyrosine, 3-Methyl-L-Mistidine, α-Amino-Adipic, Phospho-Ethanolamine, Asparagine, Phospho-L-Serine, Aspartic acid, Proline, Citrulline, Sarcosine, Glutamic acid, Argininosuccinic Acid, Glutamine, Homocitrulline. The investigators speculate that measurement of amino acids expression profiling could be taken as an indicator for assessment in critically ill patients.

Clinical Details

Official title: Amino Acids Expression Profiling of Patients With Sepsis

Study design: Observational Model: Case Control, Time Perspective: Retrospective

Primary outcome: Patients Outcome


Minimum age: 18 Years. Maximum age: N/A. Gender(s): Both.


Inclusion Criteria: 1. Male and female aged 18 years old and over; 2. Clinically confirmed infection; 3. Fulfilled at least two criteria of systemic inflammatory response syndrome (a) core temperature higher than 38 °C or lower than 36 °C (b)respiratory rate above 20/min, or PCO2 below 32 mmHg (c) pulse rate above 90/min, and (d) white blood cell count greater than 12,000/μl or lower than < 4,000/μl or less than 10% of bands. Exclusion Criteria: 1. parenteral nutrition; 2. diabetes and other metabolic-related diseases; 3. chronic liver disease; 4. neutropenia (≤ 500 neutrophils/mm3); 5. HIV infection; 6. patients or their relatives refused.

Locations and Contacts

Chinese PLA General Hospital, Beijing, Beijing 100853, China
Additional Information

Starting date: March 2012
Last updated: March 23, 2013

Page last updated: August 23, 2015

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