Immunogenicity, Efficacy and Safety Study of an MSP3-LSP (Long Synthetic Peptide) Malaria Vaccine
Information source: African Malaria Network Trust
ClinicalTrials.gov processed this data on August 23, 2015 Link to the current ClinicalTrials.gov record.
Condition(s) targeted: Malaria
Intervention: MSP3 Long Synthetic Peptide 30 micrograms of MSP3 LSP (Biological); Verorab vaccine (Biological)
Phase: Phase 2
Status: Recruiting
Sponsored by: African Malaria Network Trust Official(s) and/or principal investigator(s): Mahamadou S Sissoko, MD, MSPH, Principal Investigator, Affiliation: Malaria Research and Training Center (MRTC), Bamako Mali Roma Chilengi, MBChB, MSc, Study Director, Affiliation: African Malaria Network Trust
Overall contact: Mahamadou S Sissoko, MD, MSPH, Phone: 223-222-8109, Email: mssissoko@mrtcbko.org
Summary
This study will be the fourth time that the candidate malaria vaccine Merozoite Surface
Protein - long synthetic chain, will be tested in malaria endemic populations. in the
past,once tested in adults and twice in children proved to be safe in all three occasions
for this phase IIb study in children to proceed. This study will include children who will
be randomly allocated to either receive the malaria vaccine adjuvanted with Aluminium
Hydroxide or the Verorab control. Each participant will receive 3 immunizations, without the
clinical investigators or the participants themselves knowing what has been given. They will
then be followed-up for immediate reactions to vaccination, extended safety profile and
immunological response associated with protection from malaria. These children will be
followed up for over a longer term of two years. Blood will be taken to evaluate the
biological safety parameters and also the immune responses.
Clinical Details
Official title: Phase IIb Immunogenicity, Efficacy and Safety Study of P. Falciparum Vaccine Candidate, MSP3-LSP Adjuvanted in Aluminium Hydroxide Versus Verorab Control in Healthy Children Aged 12-48 Months in Mali.
Study design: Allocation: Randomized, Endpoint Classification: Safety/Efficacy Study, Intervention Model: Parallel Assignment, Masking: Double Blind (Subject, Caregiver, Investigator, Outcomes Assessor), Primary Purpose: Prevention
Primary outcome: Number of clinical malaria episodes occurring during the consecutive malaria transmission season after the third vaccination
Secondary outcome: Solicited adverse events measured from day 0 to day 7 after each doseUnsolicited adverse events measured up to one month after each dose Serious Adverse Events measured during the 12 months of study duration The humoral response to the vaccine antigen: assessed by measuring the level of IgG by ELISA IgG ability to recognize the native protein on Merozoite using Western Blot(WB) method Incidence of all clinical malaria episodes occurring through two transmission seasons subsequent to the 3 doses.
Detailed description:
This will be a double blind, randomized, placebo-controlled phase IIb study to evaluate the
immunogenicity, efficacy and safety of Plasmodium falciparum vaccine candidate, Merozoite
Surface Protein-3 Long synthetic peptide (MSP3) adjuvanted in aluminium hydroxide versus
Verorab control in healthy children aged 12-48 months in Mali
A phase Ib trial is currently ongoing in Burkina Faso as well as in Tanzania and its interim
results inform on the best dose/adjuvant combination to be safely extended in younger
children. The trial is evaluating immunogenicity,efficacy and safety of 3 doses of 30 µg
MSP3 adjuvanted in aluminium hydroxide
Primary objective:
- To assess the efficacy of MSP3-LSP:
- Determine the efficacy of MSP3-LSP in children aged 12-48 months against all clinical
malaria episodes (Axillary temperature of ≥ 37. 5ºC with P. falciparum parasitemia)
occurring during the consecutive malaria transmission season after the third
vaccination (six months after the third vaccination).
Secondary Objectives:
- To assess the safety and reactogenicity of 3 doses of 30 µg MSP3 adjuvanted in
aluminum hydroxide given at D0, D28 and D56 in healthy children aged 12-48 months old
in Mali.
- To assess IgG ability to recognize the native protein on Merozoite by using Western
Blot (WB) method, and measure efficacy among the subgroup of individuals able to react
with parasite proteins in WB.
- To assess the humoral immune response to the vaccine antigen using ELISA.
- Determine the efficacy of MSP3 in children aged 12-48 months against first clinical
malaria episodes.
- To assess the efficacy of MSP3-LSP in children aged 12-48 months against all clinical
malaria episodes occurring during the ensuing TWO years
Exploratory Objectives:
To further characterize the MSP3 vaccine efficacy by measuring:
- Relationship between efficacy and serological responses induced by the vaccine
- Duration of protection over the period of two years
- Vaccine efficacy against disease defined by various parasite thresholds [500, 2500,
5000, 10,000 and 20,000/µL]
- Vaccine efficacy against severe malaria disease
- Vaccine efficacy against anaemia
- To evaluate functionality of IgG by using the ADCI technique
- To assess the cellular T-helper type 1 immune responses to the vaccine antigens by
Elispot, and their persistence over 24 months of follow-up
The primary evaluation will include the following:
Solicited adverse events measured from day 0 to day 7 after each dose; Unsolicited adverse
events measured up to one month after each dose; Serious Adverse Event (SAE) measured during
the 12 months of study duration. Passive and active case detection will be used to capture
all adverse events including clinical malaria episodes. After third dose. All participants
will go through the scheduled clinic visits on days 84, 168, 365, 540 and 730 for clinical
assessment. Children will be followed for two years following the first vaccination. During
scheduled visits malaria smear and hemoglobin will be done systematically on days 0, 28, 56,
84, 168, 365, 540 and 730. The humoral immune response to the vaccine antigen will be
assessed using ELISA on days 0, 28, 56, 84, 168, 365, 540 and 730. Cellular immune response
to the vaccine antigens will be assessed on days 0, 56, 84, 168, 540 and 730 using Elispot
to MSP3-LSP. The functionality of the induced immune responses using Western Blot (WB)
method and ADCI technique will be evaluated on days 0, 84, 168, 365, 540 and 730.
Biological safety: two and four weeks after each vaccination, and thereafter every 12 weeks,
in reference with the baseline before the first dose, by measuring the following RBC,
hemoglobin, hematocrit, platelets, WBC with differential counts, ASAT, ALAT, total
bilirubin, alkaline phosphatase, γGT, creatinin.
Statistical methods:
Descriptive methods shall be employed to evaluate the above criteria.
Eligibility
Minimum age: 12 Months.
Maximum age: 48 Months.
Gender(s): Both.
Criteria:
Inclusion Criteria:
- Children aged 12-48 months old
- Healthy by medical history, physical examination and laboratory investigation
- Signed/thumb printed informed Consent by guardian/parent
- Resident in the study area villages during the whole trial period
Exclusion Criteria:
- Symptoms, physical signs of disease that could interfere with the interpretation of
the trial results or compromising the health of the subjects
- Immunosuppressive therapy (steroids, immune modulators or immune suppressors) within
3 months prior recruitment. (Inhaled and topical steroids are allowed).
- Cannot be followed for any social, psychological or geographical reasons.
- Use of any investigational drug or vaccine other than the study vaccine within 30
days preceding the first dose of study vaccine, or planned use up to 30 days after
the third dose.
- Suspected or known hypersensitivity to any of the vaccine components or to previous
vaccine.
- Laboratory abnormalities on screened blood samples.
- Planned administration of a vaccine not foreseen by the study protocol within 30 days
before the first dose of vaccine. An exception, is the receipt of an EPI or licensed
vaccine (measles, oral polio, Hib, meningococcal and combined
diphtheria/pertussis/tetanus vaccines) which may be given 14 days or more before or
after vaccination
- Evidence of chronic or active hepatitis B or C infection
- Presence of chronic illness that, in the judgment of the investigator, would
interfere with the study outcomes or pose a threat to the participant's health.
- Administration of immunoglobulin and/or any blood products within the three months
preceding the first dose of study vaccine or planned administration during the study
period
- History of surgical splenectomy.
- Moderate or severe malnutrition at screening defined as weight for age Z-score less
than 2
Locations and Contacts
Mahamadou S Sissoko, MD, MSPH, Phone: 223-222-8109, Email: mssissoko@mrtcbko.org
Malaria Research Training Center, Bamako BP 1805,point G, Mali; Recruiting Mahamadou S Sissoko, MD, MSPH, Principal Investigator
Additional Information
Starting date: May 2008
Last updated: May 6, 2008
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